Which of the following is the substrate in the enzyme assay?
A. 0.5% Azocasein
B. 2mg/mL Trypsin
C. 0.15M NaCl + 20mM Tris-HCI solution
D. 10% TCA

Answers

Answer 1

Answer:

It is 0.5 Azocasein it is not the rest

Answer 2

The 0.5 % Azocasein is the substance used in enzyme assay. So the correct option is A.

What is an enzyme substrate?

A molecule with which an enzyme interacts is known as a substrate. A substrate is put into the active site of the enzyme, which is where weak bonds between two molecules might form. The substrate reacts under the pressure of the enzyme, forming an enzyme-substrate complex that is the product of the intended reaction.

The bonds that form between the substrate and the enzyme are what induce the conformational shift, or shape change, in the enzyme. The resulting shapeshift, which either pulls molecules together or splits them apart, is what exerts pressure on the substrate.

A substrate is a material that an enzyme uses in an enzymatic process. By reducing the activation energy required for the process, enzymes operate as biological catalysts to speed up chemical reactions.

In a chemical reaction, an enzyme is a protein that catalyzes the transformation of a substrate reactant into a product. In general, individual enzymes have a variety of substrates and may be tailored to a variety of reaction intermediates that are a part of a larger process.

Therefore the correct option is A.

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Related Questions

why is the earths atmosphere likened to a greenhouse?

Answers

Answer:

That's because the glass walls of the greenhouse trap the Sun's heat. The greenhouse effect works much the same way on Earth. Gases in the atmosphere, such as carbon dioxide, trap heat similar to the glass roof of a greenhouse. These heat-trapping gases are called greenhouse gases. earth's atmosphere is like the glass in a greenhouse because sunlight streams through the atmosphere and heats the Earth. As the heat radiates up from the Earth's surface, some escapes into space. The rest of the heat is absorbed by gases in the troposphere and warms the air.Greenhouse gases include carbon dioxide, methane, nitrous oxides, and water vapor. (Water vapor, which responds physically or chemically to changes in temperature, is called a "feedback.") Scientists have determined that carbon dioxide's warming effect helps stabilize Earth's atmosphere.greenhouse gases in the atmosphere absorb some infrared radiation and re-reflect it back to earth. 6. This causes the green house effect and results in an increase in average mean temperatures on earth.Greenhouse gases include carbon dioxide, methane, nitrous oxides, and water vapor. (Water vapor, which responds physically or chemically to changes in temperature, is called a "feedback.") Scientists have determined that carbon dioxide's warming effect helps stabilize Earth's atmosphere

Explanation:

Greenhouse gases include carbon dioxide, methane, nitrous oxides, and water vapor. (Water vapor, which responds physically or chemically to changes in temperature, is called a "feedback.") Scientists have determined that carbon dioxide's warming effect helps stabilize Earth's atmosphere

PLEASE HELP DUE SOON! IM SO CONFUSED!
Write a brief story about Timmy, who accidentally walked into a rose bush, describing the reflex arc in biology 30 level detail.

Answers

When Timmy steps on the rose bush, the sensory receptor on his toe receives the stimuli, sends it the spinal cord through a sensory neuron which they initiates a response sent through a motor neuron to the muscle and he withdraws his leg in pain.

What is a reflex arc?

A reflex arc refers to the neuronal pathway which controls a reflex action.

A reflex action is an action which occurs involuntarily.

Examples of reflex actions include thhe following:

withdrawing the foot quickly when one steps on a sharp objectwithdrawing the hand quickly when one touches a hot surfacethe je_rking of the kneecoughing or sneezing, when irritants enter the nostrils.

Reflex actions do not need to involve the brain, hence they occur very quickly.

The path of a reflex arc begins with a sensory receptor which receives the stimuli, travels to the spinal cord  through a sensory neuron which they initiates a response that is sent through a motor neuron to an effector such effector such as a skeletal muscle.

In conclusion, a reflex arc is the path of a reflex action through the body.

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What is different between plant and animals

Answers

The difference between plant and animals is that plants take carbon dioxide while animals breathe in that oxygen and breathe back out carbon dioxide

Jamal and Sarah are investigating osmosis in potatoes. The drawing shows their *
experimental setup. They immersed a firm, freshly peeled potato into a container
with a solution provided by their science teacher, Ms. Stewart (Fig. 1). The next
day, they observed that the potato had shrunk and softened (Fig. 2). Describe the
nature of Ms. Stewart's solution and why the potato shrank?
Fig. 1
Your answer
24 hours
Fig. 2

Answers

The result of the experiment suggests that Ms. Stewart's solution was hypertonic, and that the potato shrunk because water moved by osmosis in favor of its concentration gradient from the potato to the solution.

What is osmosis?

Osmosis is a simple diffusion transport that occurs when water moves from a more diluted solution to a less diluted one through a semipermeable membrane.

When two solutions of different concentrations are separated by a membrane (with the same properties as the cell membrane), the only molecule allowed to pass from one side to the other is water (not solute molecules).

Water goes from the hypotonic solution (less concentrated) to the hypertonic one (more concentrated), and keeps doing so until concentrations on both sides get equal.

In the exposed example,

A fresh potato was placed on a solution of unknown solute concentration.

There were two options for this potato,

If the solution was hypotonic to the potato, the potato would have gained water. This means that water would have moved from the solution to the potato, and this last one would have swelled.

If the solution was hypertonic to the potato, the potato would have lost water. This means that water would have moved from the potato forward to the solution, and the potato would have shrunk.

After a day, Jamal and Sarah noticed that the potato shrunk.

This result suggests that Ms. Stewart's solution was hypertonic and that the potato shrunk because water moved by osmosis in favor of its concentration gradient from the potato to the solution.

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Which claim is most likely based on pseudoscience?
OA. The growth of plants is affected by the availability of light and
water.
B. Gardening is good for the soul because plants represent the
beauty of nature.
OC. Plants are often used during certain religious ceremonies.
OD. Seeds should be planted during specific moon cycles to achieve
their potential.

Answers

Answer:

D. Seeds should be planted during specific moon their cycle to achieve their potential.

Which two areas are covered in the study of physical science?
A. geology and chemistry
B.
geology and astronomy
C. physics and mathematics
D. chemistry and physics

Answers

Answer:

D

Explanation:

chemistry and physics

The Green Revolution changed agricultural methods and
allowed farmers to produce more food than ever before.
However, many scientists argue that these methods are
not sustainable.
Which action would be most likely to limit future developments in science and
engineering related to agriculture?
OA. People vote to remove government funding for research about
genetically modified crops.
B. Private companies decide to provide more money to study the use
of pesticides on crops.
C. Governments remove restrictions on research regarding
genetically modified food.
D. Scientists publish more evidence in support of modern farming
methods.

Answers

The action that would  be most likely to limit future developments in science and engineering related to agriculture People vote to remove government funding for research about genetically modified crops. Option A

What is the Green Revolution?

We would all agree that the increase in the agricultural output is the basis of the Green revolution. Food production and food sufficiency have always been at the bedrock of the development of any economy even in the developed countries.

Science and technology have become massive applied in Agriculture in order to increase the tendency of many countries towards food sufficiency.  The action that would limit future developments in science and engineering related to agriculture is; people vote to remove government funding for research about genetically modified crops.

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how do the hypothalamus and pituitary gland interact to control the release of the some of the hormones in the endocrine system ​

Answers

Answer:

Your hypothalamus communicates with the anterior pituitary lobe via hormones and the posterior lobe through nerve impulses. Through the stalk.

To vaporize water, which means turning liquid water into a
the ___
bonds between water molecules must be
This requires a lot of heat ______, which is great for
life on Earth, as it means most water is found as a

Answers

The hydrogen bonds between molecules must be broken.
This requires a lot of heat energy

Click the link below to access the prompt options for this writing task. Review the 8 prompt choices, and then choose which ONE prompt you will respond to for this essay. Prompt Choices: Genetics and Ethics Argumentative Essay Prompts.pdf After choosing your prompt, copy/paste the following questions and type your responses in the box below. 1) Which prompt are you choosing for this writing assignment? 2) Who is the audience for your argumentative report? 3) How many paragraphs should your report be? 4) True/False. You should cite reliable sources (like .edu, .org) in your report.

Answers

Answer:

ghylffffffffffffffffffffffffffffffffffffffff,ffffffffffffffffffffffffffffffffnhk

Explanation:

Topic - drug testing

Answers

Employers can determine if job candidates or current workers use drugs, including prescription medications, illegal substances, and alcohol, by conducting workplace drug tests.

Drug testing can be important for jobs of athletes, police officers, intelligence officers, surgeons and other medical staff etc.

Pros and Cons of workplace drug-testing:

Pros

Encourage your staff to take on more responsibilities.Determine which workers require assistance.Improve the workplace's health and safety.

Cons

Drug testing at work can't really be utilized as a safety precaution because they don't track drug use in real time. When they are aware that they will be subject to a drug test, many job applicants just stop using drugs.Drug tests are not always reliable.

A business should take into account the following four important aspects when thinking about any employment screening program:

costrisk reductionspeedcandidate experienceBusinesses that emphasize risk reduction should seriously consider implementing a drug-free workplace policy. Drug testing can shield workers from harm and boost output in addition to being beneficial in preventing accidents, health problems and costs, absenteeism, and litigation.Drug testing, however, can also be pricey, slow down the hiring process, and have a bad impact on the candidate experience.

If someone has a false positive drug test and they are confident that they don't do drugs, then they should be given another chance on the basis of their past medical history.

The company should bear the costs of the test since it was in their policy for all the candidates to undergo the test. Exceptions can be made based on their past medical record.

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what is skeletal system?

Answers

A system that provides structural strength and support and forms the framework of our body is known as skeletal system.

Answer:

The skeletal system is that related to the bones of our body. It means skeleton. It helps to give a full structure of our body. If there was no skeletal system then we can't stand, write, hold, walk, etc. that are needed for our growth. So, skeletal system is vital for our body to be strong.

Which scenario is an example of making inferences based on observations?
OA. A scientist collects honeybee specimens from each part of the life
cycle and creates a display.
OB. A scientist talks to various organizations about the importance of
preserving habitats vital to the survival of bees.
OC. A scientist publishes an article encouraging local farmers to plant
wildflowers to benefit wild bee populations.
D. A scientist analyzes the feeding patterns of honeybees and
determines that honeybees prefer nectar over pollen.

Answers

The example of making inferences based on observations is:

A scientist analyzes the feeding patterns of honeybees and determines that honeybees prefer nectar over pollen; option D

What are inferences and observations?

In the scientific method, observations and inferences are key step which help in understanding and explanation of the natural world.

Observation refers to the ability of the scientist to note and see changes or phenomena that occurs i his or her environment.

Inferences are conclusions which are drawn from either observations or experiments.

Considering the given scenarios, the instance of an observation is;

A scientist analyzes the feeding patterns of honeybees and determines that honeybees prefer nectar over pollen.

In conclusion, observations are required in making of inferences.

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The stimulus of a temperature change would NOT cause which of the following responses in a fish? Swimming to a different location Growing extra gills Change in activity level Change in eating habits

Answers

Answer:

Growing extra gills

Explanation:

That's impossible

Answer:

growing extra gills is the answer

Which of the following cells is formed by meiosis?
O A. A brain cell
O B. A bacterial cell
O C. A stem cell
• D. An egg cell

Answers

Answer:

D. An egg cell

Explanation:

Meiosis is the type of cell division that creates egg and sperm cells.

∴ The only answer stated from the statement is D.

Use the Internet to find the SDS for regular bleach (sodium hypochlorite, 4-6%).
Identify each of the following items on the SDS:
Physical state
Routes of exposure and symptoms
Required protective equipment
First aid procedures
Fire-fighting measures
Chemical reactivity
Safe storage
Safe disposal
Environmental precautions and ecotoxicity
Spill cleanup procedures
Record the SDS information for each of the items listed in step 2 into Data Table 2.

Answers

The features of Safety Data Sheet (SDS) for regular bleach (sodium hypochlorite, 4-6%) is illustrated below.

What is Safety Data Sheet (SDS)?

The Safety Data Sheet (SDS) is defined as an occupational safety and health sheet used as a source of information concerning harmful chemical substances in order to avoid life threatening situations while using them.

The following can be identified in the Safety Data Sheet (SDS) for bleach (sodium hypochlorite, 4-6%).

Physical state: clear light yellow green liquid.

Routes of exposure and symptoms: Eyes, skin, mucous membranes. Causes eye and skin irritation and possible burns.

Required protective equipment: Eyes;Wear chemical splash goggles. Skin; Wear appropriate protective gloves to prevent skin exposure. Clothing; Wear appropriate protective clothing to prevent skin exposure

First aid procedures: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid immediately.

Skin: Get medical aid immediately. Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse. Destroy contaminated shoes.

Fire-fighting measures: As in any fire, wear a self-contained breathing apparatus in pressure-demand and full protective gear.

Chemical reactivity: Sodium hypochlorite solutions decompose slowly at normal temperatures releasing low concentrations of corrosive chlorine gas. Decomposition is influenced by temperature, concentration, pH, ionic strength, exposure to light and the presence of metals.

Safe storage: Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances.

Safe disposal: Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste.

Environmental precautions and ecotoxicity

Environmental precautions and ecotoxicitySpill cleanup procedures: It has not been proven to be a treat to the environment.

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Jasper has an interest in a job that allows him to be on the move. Jobs like drivers, pilots, and captains of vehicles, planes, or boats are all of
Interest to Jasper. Based on this, what career pathway will Jasper MOST LIKELY pursue?
OA
Sales & Service
OB. Health, Safety & Environmental Management
OC Logistics, Planning and Management Services
OD. Transportation and Operations
Reset
Next

Answers

Transportation and operations?

Answer:

Transportation and Operations

Explanation:

I took the test

The primary cell wall surrounds the cell, and is composed of

Answers

Answer:

Cell wall is composed of cellulose.

Explanation:

*_*

Explain why the researcher chose to use the quadrat method rather than
mark and recapture to sample the coral population.

Answers

The researcher chose to use the quadrat method rather than mark and recapture to sample the coral population because corals are sessile, which means they are immobile. For a sample that exhibits mobility, the mark recapture approach is employed. i.e., being movable.

Quadrat Method- In ecology, geography, and biology, a quadrat is a frame that is typically square and is used to isolate the standard unit of space for the study of an item's distribution over a vast region.

Mark Recapture Technique- When it is impracticable to count every person in a population, the Mark-Recapture approach is employed to estimate its size.

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Question 3 of 10
Which action would most likely lead scientists to develop new explanations
or theories about pathogens?
A. Testing a hypothesis about how certain types of pathogens
interact in a controlled setting
B. Gathering new evidence about how pathogens infect and multiply
in human bodies
C. Researching the most dangerous pathogens and the cures that
have been developed
D. Developing new treatments for illnesses caused by pathogens

Answers

Answer:

B. Gathering new evidence about how pathogens infect and multiply

in human bodies

Explanation:

Answer:

B. Gathering new evidence about how pathogens infect and multiply in human bodies.

Explanation:

Got Correct On Assist.

To produce starch, glucose molecules bond together and release a water molecule through which process?
O photosynthesis.
O cellular respiration.
O nucleotides.
O dehydration synthesis.

Answers

Cellular respiration

To produce starch, glucose molecules bond together and release a water molecule through dehydration synthesis. Therefore, option (D) is correct.

To produce starch, glucose molecules undergo a process called dehydration synthesis. During dehydration synthesis, two glucose molecules bond together, and a water molecule is released as a byproduct. This reaction is also known as a condensation reaction, as the joining of two molecules results in the formation of a larger molecule and the release of water.

In the case of starch formation, multiple glucose molecules link together through dehydration synthesis, creating a long chain of glucose units, which is the structure of starch. This process is essential in the synthesis of various biological molecules, including carbohydrates, proteins, and lipids, where smaller subunits are joined together to form larger macromolecules through the removal of water molecules. Therefore, option (D) is correct.

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kinetic energy is transformed to potential energy by mitochondria and chloroplasts true or false​

Answers

kinetic energy is transformed to potential energy by mitochondria and chloroplasts TRUE

What was the calculated concentration of protein of Serum Specimen A? To receive credit,
report the value as a whole number and the units of measure!

Answers

By making use of a standard curve made up of known quantities of a pure sample protein, protein concentration is calculated. The type of chemical used can be used to separate these protein test techniques into two groups.

Either assessing the UV absorbance at 280 [tex]nm[/tex] or quantitatively reacting the protein with dyes and/or metal ions can be used to figure out the  protein concentration of sample preparation (Bradford, Lowry, or BCA assays). Simply multiply the dilution factor by the undiluted, unknown sample's protein concentration to determine its concentration. 0.5 x 10 equals 5 [tex]\frac{mg}{ml}[/tex].  By contrasting the colour produced by the "unknown" solution with the colour generated by solution of known proteins, or the standard curve, one can determine the protein content of an unknown.

The complete question is :

How to calculate the concentration of protein of Serum Unknown undiluted Specimen A? To receive credit, report the value as a whole number and the units of measure.

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What is invasive plants ?
(Write a lot and don’t use big words)

Answers

Answer:

An invasive plant is a plant that is not native to the region it is growing in. For example, English Ivy is native to Europe but has invaded the North Western coast of North America.

Explanation:

Characteristics of Invasive Plants

Invasive plants are usually very fast-growing. Their population can be hard, if not impossible to control. Additionally, like all invasive species, invasive plants place a strain on their environment. One way or another, invasive plants hurt the ecosystem they are in. This can involve the invasive plant taking up resources from native plants and/or being toxic to native animals.

We can see these characteristics in English Ivy. Like most ivies, English Ivy grows on trees and other large plants. English Ivy can become so overgrown that it weighs down the plant it is grown on. This extra weight can kill native plants. Also, due to the speed at which Ivy spreads, environmentalists have been unable to eradicate English Ivy from North America.

Causes of Invasive Plants

In almost every instance of invasive plants, the cause is human action. Through travel, humans bring seeds and plants to regions they are not native to. Then, in some cases, the plant will begin to grow rapidly and become invasive.

English Ivy was brought to North America during the Columbian Exchange.

The Columbian Exchange was the exchange of vegetation and livestock between Europe, Africa, and the Americas during the Age of Colonialism. Many invasive species can be tied back to the Columbian Exchange.

English Ivy was then used as decoration both indoors and outdoors. Since then, it has grown into a dangerous invasive plant.

Which of the following statements about electromagnetic radiation is true?
The shorter the wavelength, the more energy is carried.
The sun produces a low-energy radio waves.
The sun produces high-energy gamma rays.
All statements are correct.

Answers

Each of the claims is true. Let's look at the following as an explanation for this:

A wave's energy is directly inversely related to its frequency. As a result, as frequency increases, wavelength decreases and wave energy increases. Solar radiation, which is a subset of the vast range of energy known as the electromagnetic radiation spectrum, is how the entire amount of solar energy that reaches the Earth. Infrared, radio waves, X-rays, gamma rays, ultraviolet light, and visible light are all types of solar radiation. One technique to transport heat is by radiation.

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similarities between ecosystem approach and ecosystem management​

Answers

Explanation:

balances both human and nature's needs

protect and mange the environment

protecting the earth and its species from potential harm

The similarities between the ecosystem approach and ecosystem management are:

Balances both human and nature's needsprotect and manage the environmentprotecting the earth and its species from potential harm

What is an ecosystem approach?

The ecosystem approach is the term by which the management of the animals and plants and other living resources. The conservation or property use of these resources comes under an ecological approach.

The management of ecological services and biological resources is managed under this management system. This system also sees what extent these resources are using.

Thus, the similarities between them are:

Maintain and manage the environment.Protects the earth and its species from potential harm.Balances human and natural requirements.

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Huntington's disease is an inherited autosomal dominant disorder that can affect both men and women. Imagine a couple has had seven children, and later in life, the husband develops Huntington's disease. He is tested and it is discovered he is heterozygous for the disease allele, Hh. The wife is also genetically tested for the Huntington's disease allele, and her test results show she is unaffected, hh.

What is the percent probability that the first child of this couple will have Huntington's disease?
probability: %

What is the percent probability that four of the seven children will have Huntington's disease?
probability: %

Answers

1. The percent probability that the first child of the couple will have Huntington's disease is 50%

2. The percent probability that four of the seven children will have Huntington's disease is 6.25%

Genetical probabilities

The disease is autosomal dominant. Only one dominant allele is needed for the disease to be manifested.

The husband is Hh and the wife is hh.

            Hh    x     hh

         Hh   Hh   hh   hh

Hh (affected) = 1/2

hh (unaffected) = 1/2

1. Probability that the first child of this couple will have Huntington's disease is the same as the probability of having the disease. Thus, it is 1/2 (50%)

2. Probability that four of the seven children will have Huntington's disease is the same as the probability of having the disease in 4 places.

  1/2 x 1/2 x 1/2 x 1/2 = 1/16 or 6.25%

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3.1 What do you observe? when lighting a plastic shopping bag square​

Answers

Different amounts of light pass-through matter when it moves through it in different ways. Almost all of the light shining on transparent things can pass through them. Transparent items include glass, air, and clear plastic.

Burning plastics endangers flora, human and animal health, and emits hazardous chemicals into the environment, including dioxins, furans, mercury, and polychlorinated biphenyls (BCPs). Burning plastic releases extremely dangerous pollutants that greatly raise the risk of cancer, respiratory diseases, and birth problems. Additionally, it might seriously harm the hormonal system and internal organs. If the stench is bad, it could stay there for a few days. It might, however, improve a few hours later.

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dentify the role of the hypothesis in science

Answers

Answer:

The hypothesis is your prediction of an experiment that can disprove or corroborate the findings. It is written before the experiment and is the theory of the observation.

Explanation:

Which is NOT true about cells?

Question 4 options:

Cells are in all living things


Every type of cell performs the same function


Cells can reproduce


Cells take in food and use energy

Answers

Answer:

B

Explanation:

3 type of cells that perform different functions

Answer:

every cell type preforms the same function

Explanation:

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